RESUMEN
Biopesticides are natural compounds considered more safe and sustainable for the environment. Spinosad (SPI) is a bioinsecticide used in marketed worldwide, to eradicate a variety of pests. This study aimed to assess the impacts of the SPI on the non-target organism zebrafish (Danio rerio). Several concentrations of SPI were tested to evaluate the acute (0.07-1.0â¯mg/L) and chronic (0.006-0.100â¯mg/L) ecotoxicological effects. To evaluate sub-individual effects, antioxidant defense, lipid peroxidation, energy sources, and cholinergic biomarkers were quantified. In both exposures, SPI induced significant effects on antioxidant defense indicating oxidative stress, disrupting energy pathways, and exhibiting neurotoxic effects, under environmentally relevant conditions. Integrated Biomarker Response (IBRv2) showed that with increasing SPI concentrations, an increase in impacts on organisms was recorded. This study demonstrates the vulnerability of a non-target organism to SPI, a bioinsecticide considered environmentally safe. Further research is essential to fully understand the implications of spinosad to aquatic biota.
RESUMEN
The growing concern over the environmental impacts of industrial chemicals on aquatic ecosystems has prompted increased attention and regulation. Aromatic amines have drawn scrutiny due to their potential to disturb aquatic ecosystems. 4-chloroaniline and 3,4-dichloroaniline are chlorinated derivatives of aniline used as intermediates in the synthesis of pharmaceuticals, dyes, pesticides, cosmetics, and laboratory chemicals. While industrial applications are crucial, these compounds represent significant risks to aquatic environments. This article aims to shed light on aromatic amines' ecological and ecotoxicological impacts on aquatic ecosystems, given as examples 4-chloroaniline and 3,4-dichloroaniline, highlighting the need for stringent regulation and management to safeguard water resources. Moreover, these compounds are not included in the current Watch List of the Water Framework Directive, though there is already some information about aquatic ecotoxicity, which raises some concerns. This paper primarily focuses on the inherent environmental problem related to the proliferation and persistence of aromatic amines, particularly 4-chloroaniline and 3,4-dichloroaniline, in aquatic ecosystems. Although significant research underscores the hazardous effects of these compounds, the urgency of addressing this issue appears to be underestimated. As such, we underscore the necessity of advancing detection and mitigation efforts and implementing improved regulatory measures to safeguard the water bodies against these potential threats.
RESUMEN
Moisture is one of the major causes of problems in buildings, and it can compromise their performance. Infrared thermography (IRT) is a non-destructive testing technology that can be used to assess the humidification phenomenon and, thus, prevent some of the problems caused by moisture. The images obtained by IRT reflect the thermal patterns of the surface under study and can be evaluated using a quantitative approach, which allows not only the traditional visualization of the thermal patterns but also quantification of surface temperatures and/or their differences. The relevance of this work is related to the discussion of the strengths and weaknesses of several methods to quantitatively assess the humidification phenomenon using IRT. For that purpose, the partial humidification by the bottom surface of a lightweight concrete specimen was considered as a case study. To evaluate the thermal gradients, the evolution of the thermal imaging throughout the measurement period and the definition of the areas particularly affected by moisture, a methodology that included a pre-processing phase for data reduction, followed by a data processing phase, were implemented. In the data processing, different statistical and numerical methods were tested. The results of the statistical descriptive analysis highlighted the time variation of the surface temperature, both when considering the entire specimen and when considering only specific areas. The variability of the temperatures at certain moments of the experiment could be observed in the box-plot representation. The image subtraction proved to be an interesting technique to quantify the temperature differences if the first image was used as reference. A thermal index, TI, was proposed to assess the cooling rate. The index highlighted the initial instant when the effect of moisture on the surface temperature was detectable.
RESUMEN
Pantanal shrimp Macrobrachium pantanalense was exposed for 96 h to the cypermethrin-based formulation Barrage®. Population-relevant endpoints (survival, swimming behavior) as well as histopathology of gills were analyzed. A 96 h-LC50 of 0.93 µg/L of cypermethrin was calculated while equilibrium disturbances were observed at 1.25 µg/L. Histological examination showed predominantly regressive changes in the gills of shrimp exposed to concentrations of 0.25 and 1.25 µg/L. Three levels of lesions were observed in the gills: I- Intercellular edema, epithelial lifting of the lamellae and lamellar fusion, fat vacuoles and hypertrophy of gill epithelial cells or mucous cells; II- nuclear changes, atrophy (reduction of volume and number) and hyperplasia of gill epithelia and III- necrosis. This study shows the high sensitivity of the shrimp M. pantanalense to the pesticide Barrage® highlighting the importance of monitoring agrochemicals residues in the Pantanal region (Brazil) and conduct risk evaluation studies to prevent deleterious effects on the aquatic communities of Pantanal.
